The Stereospecificity of the Hill Reaction with Diphosphopyridine Nucleotide
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چکیده
منابع مشابه
The Stereospecificity of the Hill Reaction with Diphosphopyridine Nucleotide.
Spinach grana cause little photoreduction of DPN unless they are supplemented with ferredoxin and TPN reductase. The latter flavoprotein catalyzes a reduction of DPN+ by TPNH, and has been termed a transhydrogenase on the basis of this reaction (2, 3). The results to be presented will show that the flavoprotein transhydrogenase reaction and the Hill reaction both involve hydrogen addition to th...
متن کاملThe Stereospecificity of the Hill Reaction with Triphosphopyridine Nucleotide.
In 1957, San Pietro and Lang (1) observed that isolated spinach chloroplasts cause a light-stimulated exchange of the hydrogen of water with the hydrogen at the p position of the nicotinamide ring of oxidized TPN and DPN. Their results showed that there are enzymes in chloroplasts which cause activation of both steric positions of the reduced pyridine nucleotides. Since these early studies, it ...
متن کاملAutomated assay of activities of enzymes involving the diphosphopyridine nucleotide-reduced diphosphopyridine nucleotide reaction.
A continuous flow instrumental system of analysis, first described by Skeggs (l), was previously applied to the determination of phosphohexose isomerase activity and resulted in a method that was approximately eightfold as rapid as the manual procedure (2). The present paper is concerned with the application of this system of analysis to the determination of enzyme activities that utilize the d...
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15 صفحه اولInfluence of metallic chelates on the diphosphopyridine nucleotide oxidase and diphosphopyridine nucleotide-cytochrome c reductase systems.
The need for additional study on the specific relation between metallic ions and metabolic function, especially in the case of the diphosphopyridine nucleotide oxidase system, has been expressed by Mahler (1) and others. This is particularly indicated in view of the fact that a number of the known components of this system contain iron. While it is broadly concluded that iron compounds facilita...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97512-5